Predicting the Impact of Mutations on
Protein Solubility

SOuLMuSiC is an advanced computational tool designed to predict the impact of all possible single-site amino acid substitutions on protein solubility, based on the protein structure provided by the user.

SOuLMuSiC’s model involves a simple shallow artificial neural network that takes as input a series of features, including:

• Biophysical properties of wild-type and mutated residues
• Energetic values computed using statistical potentials
• Mutational scores derived from protein language models

Predict